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Comparison of cell culture and qPCR for detection of European North Atlantic Ranavirus from lumpfish (Cyclopterus lumpus)

TitleComparison of cell culture and qPCR for detection of European North Atlantic Ranavirus from lumpfish (Cyclopterus lumpus)
Publication TypeJournal Article
Year of Publication2021
AuthorsSigurdardottir, H, Bjornsdottir, TS, Petursson, HS, Armannsdottir, EBH, Olafsson, HG, Einarsdottir, T
JournalBull. Eur. Ass. Fish Pathol.
Volume41
Abstract

Lumpfish (Cyclopterus lumpus L.) are used as an environmentally friendly, sustainable method to delouse Atlantic salmon (Salmo salar L.). In 2014, a novel ranavirus was discovered from lumpfish. While the virus does not generally appear to be associated with disease in lumpfish, broodfish are screened for the virus and roe from infected fish are discarded. In this study, we compared cell culture screening of pooled fish samples to quantitative real-time PCR (qPCR) screening for ranaviruses in lumpfish. Our results indicate that qPCR screening was approximately 10 times more sensitive than cell culture screening. Despite the additional cost of qPCR screening, this was ultimately more economically viable, as it allowed the lumpfish farmers to selectively discard roe from infected fish only.

URLhttps://eafp.org/download/2021-volume41/issue_2/41-2-44-einarsdottir.pdf
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