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Persistence of soluble antigens of Renibacterium salmoninarum in kidney samples of Arctic charr, Salvelinus alpinus, after intraperitoneal injection

TitlePersistence of soluble antigens of Renibacterium salmoninarum in kidney samples of Arctic charr, Salvelinus alpinus, after intraperitoneal injection
Publication TypeJournal Article
Year of Publication2020
AuthorsGudmundsdottir, S, Árnason, ÍÖ, Bjornsdottir, TSoley, Jónsson, SR, Bragason, BTh
JournalBulletin of the European Association of Fish Pathologists
Volume40
Issue1
Abstract
ELISA tests are widely used to monitor the status of bacterial kidney disease (BKD) in salmonid
aquaculture and wild populations. These tests detect soluble antigens of Renibacterium salmoninarum
that circulate throughout the body of the fish and hence may detect infections in tissues other than
the one sampled. Positive ELISA shows that the fish has encountered the bacterium but it does not
reveal whether the fish harbours viable bacteria at the time of sampling. In the current study, two
different preparations of soluble R. salmoninarum antigens were injected intra-peritoneally into naïve
Arctic charr, Salvelinus alpinus that was subsequently reared for 43 weeks, in order to study the fate
of the antigens in the absence of the bacterium. Pathological changes were not observed and the
overall survival was 100%. The presence of the soluble bacterial antigens was monitored by testing
kidney samples, gathered 3 days later and after 4, 11, 22, 28 and 43 weeks, in an ELISA test. OD
values declined over time and dose dependency was observed, but 43 weeks was not sufficient time
for the fish to clear all of the soluble antigens injected. At the end of the experiment, kidneys were
also sampled for bacterial isolation and DNA analysis and all samples were negative in both tests.
URLhttps://eafp.org/download/2020-volume40/issue_1/40-1-20-metin-preview.pdf
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