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Experimental challenges with Renibacterium salmoninarum in Arctic charr Salvelinus alpinus

TitleExperimental challenges with Renibacterium salmoninarum in Arctic charr Salvelinus alpinus
Publication TypeJournal Article
Year of Publication2017
AuthorsGudmundsdottir, S, Kristmundsson, A, Árnason, ÍÖ
JournalDISEASES OF AQUATIC ORGANISMS Dis Aquat Org
Volume124
Pagination21–30
Date Published03/2017
Abstract
Arctic charr Salvelinus alpinus L. is an important species in Icelandic aquaculture
and the most common wild salmonid in Iceland. A study on the course of infection with the bacterium
Renibacterium salmoninarum was conducted using 3 different challenge methods in
brackish and fresh water. Bacterial isolation, ELISA and PCR tests were used for detection of the
bacterium in multiple organ samples. In an experiment, run for 34 wk in brackish water, infection
was established by intraperitoneal injection with 5 × 106 colony forming units (CFU) fish−1. There
were external and internal symptoms of bacterial kidney disease (BKD) and mortalities between 6
and 13 wk after injection. A cohabitation trial was run simultaneously and infection was well
established after 4 wk, as demonstrated by the detection methods applied. Symptoms of BKD were
not seen and all but 1 cohabitant survived. In a separate experiment, infection was established by
pumping a fixed amount of water from a tank with fingerlings infected by intraperitoneal injection
into tanks with naïve fish, in fresh or brackish water, for 6 wk. Fish in the inflow tanks were reared
for an additional 3 wk. There were neither macroscopic symptoms nor mortalities. ELISA and PCR
tests showed that infection started to take hold after 3 wk. The challenge trials demonstrated that
Arctic charr is susceptible to R. salmoninarum. Cohabitation and inflow of water from tanks with
infected fish provide useful models for further studies on R. salmoninarum infection acquired in
a natural way in Arctic charr.
 
DOI10.3354/dao03107
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